Journal article
l-Tyrosine Confers Residualizing Properties to a d-Amino Acid-Rich Residualizing Peptide for Radioiodination of Internalizing Antibodies
FT Lee, IJG Burvenich, N Guo, P Kocovski, H Tochon-Danguy, U Ackermann, GJ O’Keefe, S Gong, A Rigopoulos, Z Liu, HK Gan, AM Scott
Molecular Imaging | Published : 2016
Abstract
Purpose: The aims of the study were to develop and evaluate a novel residualizing peptide for labeling internalizing antibodies with 124I to support clinical development using immuno-positron emission tomography (PET). Methods: The anti-epidermal growth factor receptor antibody ch806 was radiolabeled directly or indirectly with isotopes and various residualizing peptides. Azido-derivatized radiolabeled peptides were conjugated to dibenzylcyclooctyne-derivatized ch806 antibody via click chemistry. The radiochemical purities, antigen-expressing U87MG.de2-7 human glioblastoma cell-binding properties, and targeting of xenografts at 72 hours post injection of all radioconjugates were compared. Bi..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported in part by NHMRC Project grant 1003085 and NHMRC Program grant 487922. Operational Infrastructure Support Program funding was provided by the Victorian Government and funds to purchase the imaging equipment was provided by the Australian Cancer Research Foundation.